Journal: PLoS ONE
Article Title: Intervention Effects of Ganoderma Lucidum Spores on Epileptiform Discharge Hippocampal Neurons and Expression of Neurotrophin-4 and N-Cadherin
doi: 10.1371/journal.pone.0061687
Figure Lengend Snippet: Compared with the model group, the expression of NT-4 was higher in the GLS group I (GLS dissolved into Mg ion free medium and incubated for 3 hours) and II (neurons incubated with Mg ion free medium for 3 hours then incubated with GLS dissolved into the normal medium for 6 hours), and the expression of N-Cadherin was lower. Compared with the control group, the expression of NT-4 and N-Cadherin were higher in GLS group I and II.
Article Snippet: It was then incubated with a rabbit-anti-β-actin polyclonal antibody (BAB) rabbit-anti-NT-4 BAB and rabbit-anti-N-Cadherin BAB (Cat. No. is BA2305, BA1294, BA0673 respectively, Wuhan Boster Bio-Engineering Ltd, China) respectively for 2 hours at 37°C, rinsed with Tris-buffered saline with Tween 20 (TBST) 3 times for 10 minutes each, and then incubated with horse radish peroxidase-labeled goat-anti-rabbit IgG antibody for 1 hour at 37°C.
Techniques: Expressing, Incubation, Control